Olfactomedin-4 + neutrophils exacerbate intestinal epithelial damage and worsen host survival after Clostridioides difficile infection.

A Huber Shinsmon JoseA KassamK N WeghornMargaret V Powers-FletcherD SharmaA MukherjeeA MathewN KulkarniS ChandramouliMatthew N Alder Rajat Madan

Published in: bioRxiv : the preprint server for biology (2023)

Utilizing single-cell transcriptomics, IEC-epithelial co-cultures, and pre-clinical models of CDI, we have identified a subset of neutrophils that are marked by OLFM4 expression as pathogenic determinants of IEC barrier damage after CDI.

Keyphrases

single cell
rna seq
clostridium difficile
oxidative stress
poor prognosis
high throughput