An integrated workflow for crosslinking mass spectrometry.
Marta L MendesLutz FischerZhuo A ChenMarta BarbonFrancis J O'ReillySven H GieseMichael Bohlke-SchneiderAdam BelsomTherese DauColin W CombeMartin GrahamMarkus R EiseleWolfgang BaumeisterChristian SpeckJuri RappsilberPublished in: Molecular systems biology (2020)
We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate our simple 12-fraction protocol for crosslinked multi-protein complexes and cell lysates, quantitative analysis, and high-density crosslinking, without requiring specific crosslinker features. This overall approach reveals dynamic protein-protein interaction sites, which are accessible, have fundamental functional relevance and are therefore ideally suited for the development of small molecule inhibitors.
Keyphrases
- protein protein
- small molecule
- high density
- mass spectrometry
- loop mediated isothermal amplification
- label free
- electronic health record
- randomized controlled trial
- amino acid
- liquid chromatography
- anaerobic digestion
- cell therapy
- hyaluronic acid
- stem cells
- high performance liquid chromatography
- capillary electrophoresis
- quantum dots