ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines.
Petr DanielKamila BalušíkováRadka VáclavíkováKarolina SeborovaŠárka RansdorfováMarie ValeriánováLongfei WeiMichael JelínekTereza TlapákováThomas FleischerVessela N KristensenPavel SoučekIwao OjimaJan KovarPublished in: Genes (2023)
A limited number of studies are devoted to regulating TRIP6 expression in cancer. Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.
Keyphrases
- breast cancer cells
- poor prognosis
- binding protein
- metastatic breast cancer
- positive breast cancer
- transcription factor
- long non coding rna
- type diabetes
- genome wide
- copy number
- gene expression
- squamous cell carcinoma
- lymph node
- pregnant women
- metabolic syndrome
- adipose tissue
- dna methylation
- cell death
- lymph node metastasis
- childhood cancer
- energy transfer