LncRNA-SNHG5 mediates activation of hepatic stellate cells by regulating NF2 and Hippo pathway.
Rongrong ZhangYating ZhanZhichao LangYifei LiWeizhi ZhangJianjian ZhengPublished in: Communications biology (2024)
Long noncoding RNA small nucleolar RNA host gene 5 (SNHG5) is an oncogene found in various human cancers. However, it is unclear what role SNHG5 plays in activating hepatic stellate cells (HSCs) and liver fibrosis. In this study, SNHG5 was found to be upregulated in activated HSCs in vitro and in primary HSCs isolated from fibrotic liver in vivo, and inhibition of SNHG5 suppressed HSC activation. Notably, Neurofibromin 2 (NF2), the main activator for Hippo signalling, was involved in the effects of SNHG5 on HSC activation. The interaction between SNHG5 and NF2 protein was further confirmed, and preventing the combination of the two could effectively block the effects of SNHG5 inhibition on EMT process and Hippo signaling. Additionally, higher SNHG5 was found in chronic hepatitis B patients and associated with the fibrosis stage. Altogether, we demonstrate that SNHG5 could serve as an activated HSCs regulator via regulating NF2 and Hippo pathway.
Keyphrases
- poor prognosis
- signaling pathway
- long noncoding rna
- liver fibrosis
- induced apoptosis
- long non coding rna
- pi k akt
- nuclear factor
- lps induced
- oxidative stress
- cell cycle arrest
- end stage renal disease
- endothelial cells
- gene expression
- chronic kidney disease
- transcription factor
- cell proliferation
- cell death
- small molecule
- systemic sclerosis
- idiopathic pulmonary fibrosis
- genome wide