Chitosan nanoparticles as a promising candidate for liver injury induced by 2-nitropropane: Implications of P53, iNOS, VEGF, PCNA, and CD68 pathways.
Sameerah ShaheenMaha M ArafahAliah R AlshanwaniLaila Mohammed FaddaAhlam M AlhusainiHanaa M AliIman H HasanHanan HagarFatima Mb AlharbiAlaa AlHarthiiPublished in: Science progress (2022)
The current article was designed to assess the role of chitosan nanoparticles (CNPs) in the management of hepatic injury induced by the hepatocarcinogen 2-nitropropane (2-NP). Rats were divided into three groups. The first group served as a control, the second group was injected with 2-NP, while the third group was treated with CNPs 1 h before 2-NP injection every other day for 4 weeks. The 2-NP injection upregulated serum AST and ALT activities, as well as hepatic TNF- α, IL-6, and MDA levels and the expression of vascular endothelial growth factor (VEGF) and caspase-3, whereas GSH contents and SOD activity were decreased. Immunohistochemistry investigations revealed that the hepatic protein expression of collagen I, inducible nitric oxide synthetase, proliferating cell nuclear antigen, cluster of differentiation, and p53 were upregulated. hematoxylin and eosin (H&E) and Masson's trichrome stains supported the previous parameters, and CNPs ameliorated most of the previous biochemical parameters. CNPs achieved promising results in the limitation of 2-NP hepatotoxicity.
Keyphrases
- vascular endothelial growth factor
- liver injury
- drug induced
- nitric oxide
- endothelial cells
- drug delivery
- single cell
- wound healing
- poor prognosis
- rheumatoid arthritis
- cell death
- ultrasound guided
- stem cells
- cell therapy
- hyaluronic acid
- hydrogen peroxide
- fluorescent probe
- amyotrophic lateral sclerosis
- induced apoptosis