Analytical Performance Evaluation of New DESI Enhancements for Targeted Drug Quantification in Tissue Sections.
Margaux FresnaisSiwen LiangMarius BreitkopfJoshua Raoul LindnerEmmanuelle ClaudeSteven PringlePavel A LevkinKonstantin DemirJulia BenzelJulia SundheimerBritta StatzKristian W PajtlerStefan M PfisterWalter E HaefeliJuergen BurhenneRémi LonguespéePublished in: Pharmaceuticals (Basel, Switzerland) (2022)
Desorption/ionization (DI)-mass spectrometric (MS) methods offer considerable advantages of rapidity and low-sample input for the analysis of solid biological matrices such as tissue sections. The concept of desorption electrospray ionization (DESI) offers the possibility to ionize compounds from solid surfaces at atmospheric pressure, without the addition of organic compounds to initiate desorption. However, severe drawbacks from former DESI hardware stability made the development of assays for drug quantification difficult. In the present study, the potential of new prototype source setups (High Performance DESI Sprayer and Heated Transfer Line) for the development of drug quantification assays in tissue sections was evaluated. It was demonstrated that following dedicated optimization, new DESI XS enhancements present promising options regarding targeted quantitative analyses. As a model compound for these developments, ulixertinib, an inhibitor of extracellular signal-regulated kinase (ERK) 1 and 2 was used.
Keyphrases
- high throughput
- cancer therapy
- drug induced
- mass spectrometry
- multiple sclerosis
- signaling pathway
- adverse drug
- cell proliferation
- biofilm formation
- transcription factor
- protein kinase
- emergency department
- escherichia coli
- pi k akt
- staphylococcus aureus
- climate change
- human health
- single cell
- cystic fibrosis
- gas chromatography
- candida albicans