Untargeted metabolomics reveals altered branch chain amino acids, glucose and fat metabolism contributing to coronary artery disease among Indian diabetic patients.
Ramu AdelaSiva Swapna KasarlaNajmuddin SaquibSonu Kumar GuptaSneh BajpaiYashwant KumarSanjay Kumar BanerjeePublished in: Molecular omics (2023)
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterised by increased blood glucose levels. Patients with T2DM have a high risk of developing atherosclerotic coronary artery disease (CAD). CAD with T2DM has a complex etiology and the understanding of the pathophysiology of coronary artery disease (CAD) in the presence of diabetes is poor. Here, we have used LC-MS/MS-based untargeted metabolomics to unveil the alterations of metabolites in the serum of South-Indian patients diagnosed with T2DM, CAD and T2DM along with CAD (T2DM-CAD) compared with the healthy subjects (CT). Using untargeted metabolomics and network-based approaches, a set of metabolites highly co-expressed with T2DM-CAD pathogenesis were identified. Our results revealed that these metabolites belong to essential pathways such as amino acid metabolism, fatty acid metabolism and carbohydrate metabolism. The candidate metabolites identified by metabolomics study are branch chain amino acids, L-arginine, linoleic acid, L-serine, L-cysteine, fructose-6-phosphate, glycerol, creatine and 3-phosphoglyceric acid, and explain the pathogenesis of T2DM-assisted CAD. The identified metabolites could be used as potential prognostic markers to predict CAD in patients diagnosed with T2DM.
Keyphrases
- coronary artery disease
- glycemic control
- blood glucose
- amino acid
- mass spectrometry
- percutaneous coronary intervention
- cardiovascular events
- coronary artery bypass grafting
- end stage renal disease
- ms ms
- type diabetes
- newly diagnosed
- fatty acid
- chronic kidney disease
- peritoneal dialysis
- liquid chromatography
- cardiovascular disease
- nitric oxide
- skeletal muscle
- acute coronary syndrome
- contrast enhanced
- gas chromatography
- protein kinase