Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases.

Published in: Methods in molecular biology (Clifton, N.J.) (2022)

Here, we describe a protocol for reprogramming of bone marrow-derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease.

Keyphrases

bone marrow
stem cells
induced pluripotent stem cells
mesenchymal stem cells
randomized controlled trial
induced apoptosis
cell therapy
signaling pathway
high glucose
oxidative stress
endoplasmic reticulum stress
stress induced