Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation.

An early-developmental time course of zebrafish chromatin accessibility is achieved using an integrated UMAP analysis of datasets from two separate published studies.CUT&Tag sequencing is used to characterize the genomic localization for the histone chaperone ANP32E.Changes in Anp32e enrichment coincide with opposing changes in H2A.Z enrichment during zebrafish gastrulation.Precociously expressed genes in embryos lacking Anp32e are disproportionately Sox-marked and may represent H2A.Z-mediated developmental accelerations.