MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients.
Andrea SantosIon CristóbalJaime RubioCristina CaramésMelani LuqueMarta Sanz-AlvarezMiriam Morales-GallegoJuan Madoz-GúrpideFederico RojoJesús García-FoncillasPublished in: International journal of molecular sciences (2022)
The identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reported to predict outcome and response to neoadjuvant chemoradiotherapy (nCRT) in LARC, and was also found to be associated with disease progression in colorectal cancer. However, its biological and clinical relevance remains to be fully clarified. Thus, we observed here that miR-199b regulates cell migration, aggressiveness, and cell growth, and inhibits colonosphere formation and induces caspase-dependent apoptosis. Moreover, miR-199b expression was quantified by real-time PCR in plasma samples from LARC patients and its downregulation was observed in 22.7% of cases. This alteration was found to be associated with higher tumor size ( p = 0.002) and pathological stage ( p = 0.020) after nCRT. Notably, we observed substantially lower global miR-199b expression associated with patient downstaging ( p = 0.009), as well as in non-responders compared to those cases who responded to nCRT in both pre- ( p = 0.003) and post-treatment samples ( p = 0.038). In concordance, we found that miR-199b served as a predictor marker of response to neoadjuvant therapy in our cohort ( p = 0.011). Altogether, our findings here demonstrate the functional relevance of miR-199b in this disease and its potential value as a novel circulating marker in LARC.
Keyphrases
- rectal cancer
- locally advanced
- neoadjuvant chemotherapy
- cell migration
- poor prognosis
- phase ii study
- end stage renal disease
- squamous cell carcinoma
- real time pcr
- radiation therapy
- cell death
- chronic kidney disease
- ejection fraction
- oxidative stress
- prognostic factors
- signaling pathway
- cell proliferation
- clinical trial
- peritoneal dialysis
- lymph node
- bone marrow
- stem cells
- induced apoptosis
- patient reported outcomes
- patient reported