Automated Decellularization of the Rodent Epigastric Free Flap: A Comparison of Sodium Dodecyl Sulfate-Based Protocols.

Fuat Barış BengürLei ChenBenjamin K SchillingChiaki KomatsuGrace M FiglioliKacey G MarraLauren E KokaiMario G Solari

Published in: Journal of reconstructive microsurgery (2022)

A compact and integrated bioreactor can automate decellularization of free flaps to bioengineer regenerative constructs for future use in reconstruction of complex defects. A decellularization protocol with 5 days of 1% SDS exposure was the most successful to keep the residual DNA content at a minimum while preserving the structural integrity of the tissues.

Keyphrases

stem cells
breast reconstruction
mesenchymal stem cells
randomized controlled trial
gene expression
wastewater treatment
circulating tumor
deep learning
high throughput
cell therapy
cell free
single molecule
soft tissue
nucleic acid