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Evaluation and minimization of nonspecific tryptic cleavages in proteomic sample preparation.

Zhilong LinYan RenZhaomei ShiKeren ZhangHuanming YangSiqi LiuPiliang Hao
Published in: Rapid communications in mass spectrometry : RCM (2021)
The significant decrease in nonspecific cleavages and artificial modifications improves the accuracy of protein quantification and the identification of low-abundance proteins, and it is especially useful for studying protein posttranslational modifications. For trypsin digestion, the proposed 2-h protocol can potentially be a replacement for the traditional 16-h protocol.
Keyphrases
  • randomized controlled trial
  • protein protein
  • amino acid
  • binding protein
  • small molecule
  • label free