Evaluation and minimization of nonspecific tryptic cleavages in proteomic sample preparation.
Zhilong LinYan RenZhaomei ShiKeren ZhangHuanming YangSiqi LiuPiliang HaoPublished in: Rapid communications in mass spectrometry : RCM (2021)
The significant decrease in nonspecific cleavages and artificial modifications improves the accuracy of protein quantification and the identification of low-abundance proteins, and it is especially useful for studying protein posttranslational modifications. For trypsin digestion, the proposed 2-h protocol can potentially be a replacement for the traditional 16-h protocol.