Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine-Shell Nanoprobe.
Wai Ki WongSiu Hong Dexter WongLiming BianPublished in: ACS biomaterials science & engineering (2020)
Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines in vitro. A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.
Keyphrases
- living cells
- papillary thyroid
- fluorescent probe
- early stage
- loop mediated isothermal amplification
- cell proliferation
- squamous cell
- long non coding rna
- label free
- real time pcr
- transcription factor
- poor prognosis
- squamous cell carcinoma
- long noncoding rna
- palliative care
- lymph node metastasis
- binding protein
- quantum dots
- sentinel lymph node
- sensitive detection
- amino acid