MicroRNA expression profile in Lampetra morii upon Vibrio anguillarum infection and miR-4561 characterization targeting lip.
Lie MaMeng GouZeyu DuTing ZhuJun LiQing Wei LiYue PangPublished in: Communications biology (2021)
As a critical evolutionary pivot between invertebrates and vertebrates, lampreys provide rich genetic information. Lamprey immune protein (LIP) is a key immune regulator. MicroRNAs, well-conserved in the response to immunological stress, remain understudied in lamprey immunity. We generated a lamprey microRNA expression atlas, using deep sequencing, upon Vibrio anguillarum infection. Using comparative methods, we found that miR-4561 potentially regulates innate immunity via interaction with lip. We found a sequence in the 3'-UTR region of LIP mRNA complementary to the miR-4561 seed region; miR-4561 expression was negatively correlated with LIP. During V. anguillarum infection, miR-4561 inhibited LIP expression and bacterial clearance. Notably, LIP expression in supraneural body cells was necessary for the Gram-negative immune response. Additionally, we observed that overexpression of miR-4561 induced apoptosis in embryonic cells, suggesting a role in embryonic development. Collectively, we show lamprey microRNAs may significantly affect gene regulation and provide new insights on LIP-mediated immune regulation.
Keyphrases
- long non coding rna
- induced apoptosis
- cell proliferation
- poor prognosis
- long noncoding rna
- endoplasmic reticulum stress
- immune response
- signaling pathway
- gram negative
- binding protein
- multidrug resistant
- transcription factor
- cell cycle arrest
- healthcare
- escherichia coli
- pi k akt
- gene expression
- small molecule
- cancer therapy
- staphylococcus aureus
- dendritic cells
- toll like receptor
- copy number
- biofilm formation
- candida albicans