CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor.
Ling WanJianfeng MaJiasheng YiYan DongRenjie NiuYan SuQian Linull Dan ZhuJie ChaoShao SuChun-Hai FanLian-Hui WangYing WanPublished in: Chemical communications (Cambridge, England) (2022)
Rapid pathogen screening holds the key against certain viral infections, especially in an overwhelming pandemic. Herein, a CRISPR-empowered electrochemical biosensor was designed for the ultrasensitive detection of the avian influenza A (H7N9) virus gene sequence. Combining the CRISPR/Cas system, a signal-amplification strategy and a high-conductivity sensing substrate, the developed biosensor showed an ultrawide dynamic range, an ultralow detection limit, and excellent selectivity for H7N9 detection, providing a potential sensing platform for the simple, fast, sensitive, and on-site detection of infectious diseases.
Keyphrases
- label free
- crispr cas
- loop mediated isothermal amplification
- genome editing
- gold nanoparticles
- genome wide
- infectious diseases
- sars cov
- real time pcr
- sensitive detection
- ionic liquid
- high throughput
- quantum dots
- candida albicans
- transcription factor
- climate change
- amino acid
- liquid chromatography
- molecularly imprinted