Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis.

Arturo B RamirezRaksha BhatDebashish SahayCarmine De AngelisHariprasad ThangavelSina HedayatpourLacey E DobroleckiAgostina NardoneMario GiulianoChandandeep NagiMothaffar RimawiC Kent OsborneMichael T LewisJackie L StilwellEric P KaldjianRachel SchiffMeghana V Trivedi

Published in: BMC cancer (2019)

Analysis of small volume blood samples from BC-PDX-bearing mice using the AccuCyte-CyteFinder system allows investigation of the role of CTCs in tumor biology and metastasis independent of surface marker expression.

Keyphrases

single cell
circulating tumor
circulating tumor cells
high throughput
deep learning
rna seq
poor prognosis
cell free
machine learning
high fat diet induced
type diabetes
adipose tissue
long non coding rna
mesenchymal stem cells
young adults
insulin resistance
data analysis