Deciphering the sex bias in housekeeping gene expression in adipose tissue: a comprehensive meta-analysis of transcriptomic studies.

Maria Guaita-CespedesRubén Grillo-RiscoMarta R HidalgoSonia Fernández-VeledoDeborah Jane BurksMaría de la Iglesia-VayáAmparo GalánFrancisco García-García

Published in: Biology of sex differences (2023)

This sex-based research proves that certain classical housekeeping genes fail to function adequately as controls when analyzing human adipose tissue considering sex as a variable. We confirm RPL19 and PPIA suitability as sex-unbiased human and mouse housekeeping genes derived from sex-specific expression profiles, and propose new ones such as RPS8 and UBB.

Keyphrases

adipose tissue
gene expression
endothelial cells
high fat diet
genome wide
induced pluripotent stem cells
pluripotent stem cells
dna methylation
genome wide identification