Densazalin, a New Cytotoxic Diazatricyclic Alkaloid from the Marine Sponge Haliclona densaspicula.
Buyng Su HwangYong Tae JeongSangbum LeeEun-Ju JeongJung-Rae RhoPublished in: Molecules (Basel, Switzerland) (2021)
Densazalin, a polycyclic alkaloid, was isolated from the marine sponge Haliclona densaspicula collected in Korea. The complete structure of the compound was determined by spectroscopic methods, including 1D and 2D nuclear magnetic resonance techniques, high-resolution mass spectrometry, and comparison of the calculated and measured electronic circular dichroism spectra. Densazalin possesses a unique 5,11-diazatricyclo[7.3.1.02,7]tridecan-2,4,6-triene moiety, which is connected by two linear carbon chains. This compound was derived from the biogenetic precursor bis-1,3-dialkylpyridnium. Densazalin exhibited cytotoxic activity on two human tumor cell lines (AGS and HepG2) in the Cell Counting Kit-8 (CCK-8) bioassay, with IC50 values ranging from 15.5 to 18.4 μM.
Keyphrases
- high resolution mass spectrometry
- magnetic resonance
- liquid chromatography
- endothelial cells
- ultra high performance liquid chromatography
- single cell
- gas chromatography
- molecular docking
- mass spectrometry
- cell therapy
- induced pluripotent stem cells
- pluripotent stem cells
- tandem mass spectrometry
- ionic liquid
- density functional theory
- stem cells
- molecular dynamics