A Low-Field Magnetic Resonance Imaging Aptasensor for the Rapid and Visual Sensing of Pseudomonas aeruginosa in Food, Juice, and Water.
Fei JiaXingjian BaiXiaowei ZhangYingchun FuYanbin LiXingmin LiJozef L KokiniPublished in: Analytical chemistry (2021)
In this work, we present a low-field magnetic resonance imaging (LF-MRI) aptasensor based on the difference in magnetic behavior of two magnetic nanoparticles with diameters of 10 (MN10) and 400 nm (MN400) for the rapid detection of Pseudomonas aeruginosa (P. aeruginosa). First, specific anti-P. aeruginosa aptamers were covalently immobilized onto magnetic nanoparticles via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide chemistry for the capture of the target bacteria. In the presence of P. aeruginosa, an MN10-bacteria-MN400 (MBM) complex was formed after binding between the aptamers on magnetic nanoparticles and P. aeruginosa cells. When a magnetic field was applied, the MBM complex and free MN400 were rapidly magnetically separated, and free MN10 left in the solution worked as a T2 (transverse relaxation time) single readout in MRI measurement. Under optimum conditions, the LF-MRI platform provides both image analysis and quantitative detection of P. aeruginosa, with a detection limit of 100 cfu/mL. The feasibility and specificity of the aptasensor were demonstrated in detecting real food, orange juice, and drinking water samples and validated using plate counting methods.
Keyphrases
- photodynamic therapy
- magnetic nanoparticles
- magnetic resonance imaging
- contrast enhanced
- pseudomonas aeruginosa
- label free
- room temperature
- transition metal
- loop mediated isothermal amplification
- sensitive detection
- metal organic framework
- quantum dots
- diffusion weighted imaging
- cystic fibrosis
- computed tomography
- induced apoptosis
- acinetobacter baumannii
- ionic liquid
- magnetic resonance
- staphylococcus aureus
- oxidative stress
- human health
- high resolution
- high throughput
- drug resistant
- cell proliferation
- single cell
- single molecule
- molecularly imprinted
- nucleic acid
- capillary electrophoresis