By introducing compulsory antiseptic measures in 1881, the Austrian Empire became a trendsetter in European midwifery legislation. Starting with the focus on puerperal infection in the 1870s, this article investigates the process from the first proposal of antiseptic regimes within a clinical setting to the dissemination of antiseptic knowledge among the midwifery profession. Competition between the leading medical men throughout the Austrian territories played a major role and influenced the way in which antiseptic measures were propagated. The article identifies the antiseptic collectives active at the leading universities of Prague and Vienna. As practical instruction during midwifery education was not regarded as sufficient, the late 1870s and 1880s saw the emergence of several instruction textbooks. Changing birth attendance routines and the innovative materials that entered midwifery practice are explored and discussed, based on these manuals, alongside evidence of midwives' reactions as published in the Austrian Midwifery Newspaper.