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A new, simple, highly scalable, and efficient protocol for genomic DNA extraction from diverse plant taxa.

Evgeny V MavrodievChristopher DervinisWilliam Mark WhittenMatthew A GitzendannerMatias KirstSangtae KimTaliesin J KinserPamela S SoltisDouglas E Soltis
Published in: Applications in plant sciences (2021)
Application of the protocol to various plant species yielded DNA of high quality in less than two hours and can be adjusted to extract DNA at large (maxi-preps) or small (96-well minipreps) scales. We anticipate that our method will be of wide utility for rapidly isolating large quantities of quality genomic DNA from diverse plant species and will have broad applications in phylogenetic studies utilizing PCR and short-read DNA sequencing.
Keyphrases
  • circulating tumor
  • single molecule
  • cell free
  • randomized controlled trial
  • nucleic acid
  • oxidative stress
  • copy number
  • dna methylation
  • quality improvement