A pulsed current electric field alters protein expression creating a wound healing phenotype in human skin cells.
Luciana E BostanSofia AlmqvistChristine E PullarPublished in: Regenerative medicine (2020)
Aim: Pulsed current (PC) electric field (EF) devices promote healing in chronic wounds but the underpinning mechanisms are largely unknown. The gap between clinical evidence and mechanistic understanding limits device uptake in clinics. Materials & methods: Migration, proliferation and gene/protein expression profiles were investigated in the presence/absence of PCEF, in skin: keratinocytes (NHK); dermal fibroblasts (HDF); dermal microvascular endothelial cells (HDMEC) and macrophages (THP-1). Results: While PCEF had little effect on migration or proliferation, it significantly altered the expression of 31 genes and the secretion of 7 pro-angiogenic and pro-regenerative growth factors using ELISAs. Conclusion: PCEF significantly altered skin cell genomes/proteomes which provides some evidence of how PCEF devices promote healing of chronic wounds.
Keyphrases
- wound healing
- endothelial cells
- signaling pathway
- induced apoptosis
- cell therapy
- genome wide
- stem cells
- mesenchymal stem cells
- genome wide identification
- poor prognosis
- anti inflammatory
- primary care
- single cell
- cell cycle arrest
- binding protein
- copy number
- oxidative stress
- drug induced
- genome wide analysis
- gene expression
- atomic force microscopy
- amino acid
- tissue engineering
- cell proliferation
- long non coding rna
- protein protein