Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten.

Shalima S NairPhuc-Loi LuuWenjia QuMadhavi MaddugodaLily HuschtschaRoger ReddelGeorgia Chenevix-TrenchMartina TosoJames G KenchLisa G HorvathVanessa M HayesPhillip D StrickerTimothy P HughesDeborah L WhiteJohn E J RaskoJustin J-L WongSusan J Clark

Published in: Epigenetics & chromatin (2018)

Together we provide a systematic, efficient and complete approach to perform and analyse WGBS on the HiSeq X Ten. Our protocol allows for large-scale WGBS studies at reasonable processing time and cost on the HiSeq X Ten platform.

Keyphrases

randomized controlled trial
circulating tumor
high throughput
single cell
single molecule
cell free
clinical practice
case control