Cardiac Alarmins as Residual Risk Markers of Atherosclerosis under Hypolipidemic Therapy.
Viorel-Iulian SuicaElena UyyLuminita IvanRaluca M BoteanuAurel Cerveanu-HogasRune HansenFelicia AntohePublished in: International journal of molecular sciences (2022)
Increased levels of low-density lipoproteins are the main risk factor in the initiation and progression of atherosclerosis. Although statin treatment can effectively lower these levels, there is still a residual risk of cardiovascular events. We hypothesize that a specific panel of stress-sensing molecules (alarmins) could indicate the persistence of silent atherosclerosis residual risk. New Zealand White rabbits were divided into: control group (C), a group that received a high-fat diet for twelve weeks (Au), and a treated hyperlipidemic group with a lipid diet for eight weeks followed by a standard diet and hypolipidemic treatment (atorvastatin and PCSK9 siRNA-inhibitor) for four weeks (Asi). Mass spectrometry experiments of left ventricle lysates were complemented by immunologic and genomic studies to corroborate the data. The hyperlipidemic diet determined a general alarmin up-regulation tendency over the C group. A significant spectral abundance increase was measured for specific heat shock proteins, S100 family members, HMGB1, and Annexin A1. The hypolipidemic treatment demonstrated a reversed regulation trend with non-significant spectral alteration over the C group for some of the identified alarmins. Our study highlights the discriminating potential of alarmins in hyperlipidemia or following hypolipidemic treatment. Data are available via ProteomeXchange with identifier PXD035692.
Keyphrases
- high fat diet
- cardiovascular disease
- cardiovascular events
- physical activity
- coronary artery disease
- heat shock
- weight loss
- adipose tissue
- big data
- left ventricular
- type diabetes
- heart failure
- magnetic resonance
- pulmonary hypertension
- magnetic resonance imaging
- risk factors
- insulin resistance
- optical coherence tomography
- metabolic syndrome
- mitral valve
- drug delivery
- oxidative stress
- dna methylation
- machine learning
- pulmonary artery
- genome wide
- high performance liquid chromatography
- wastewater treatment
- combination therapy
- artificial intelligence
- pulmonary arterial hypertension
- electronic health record
- cancer therapy
- replacement therapy
- risk assessment
- preterm birth
- ms ms
- fatty acid
- dual energy
- tandem mass spectrometry