Login / Signup

An identification method to distinguish monomeric sugar isomers on glycopeptides.

Ashley E DeYongJonathan C TrinidadNicola L B Pohl
Published in: The Analyst (2023)
A one-step protocol for the automated flow synthesis of protected glycosylated amino acids is described using pumps with open-source controls in overall yields of 21-50%. The resulting glycosylated amino acids could be used directly in solid-phase peptide synthesis (SPPS) protocols to quickly produce glycopeptide standards. Access to a variety of stereoisomers of the sugar enabled the development of an LC-MS/MS protocol that can distinguish between peptides modified with carbohydrates having the same exact mass. This method could definitively identify fucose in an O -glycosylation site on the transmembrane protein, Notch1.
Keyphrases
  • amino acid
  • randomized controlled trial
  • machine learning
  • deep learning
  • high throughput
  • cell proliferation
  • density functional theory
  • bioinformatics analysis