Instrumental analysis of RNA modifications.
Yasemin YoluçGregor AmmannPierre BarraudManasses JoraPatrick A LimbachYuri MotorinVirginie MarchandCarine TisnéKayla BorlandStefanie M KellnerPublished in: Critical reviews in biochemistry and molecular biology (2021)
Organisms from all domains of life invest a substantial amount of energy for the introduction of RNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA can focus on the modified residues and reveal the function of these epitranscriptomic marks. Here, we will review recent advances and breakthroughs achieved by NMR spectroscopy, sequencing, and mass spectrometry of the epitranscriptome.