Induction of Necrosis in Human Macrophage Cell Lines by Corynebacterium diphtheriae and Corynebacterium ulcerans Strains Isolated from Fatal Cases of Systemic Infections.
Dulanthi WeerasekeraJonas HahnMartin HerrmannAndreas BurkovskiPublished in: International journal of molecular sciences (2019)
When infecting a human host, Corynebacterium diphtheriae and Corynebacterium ulcerans are able to impair macrophage maturation and induce cell death. However, the underlying molecular mechanisms are not well understood. As a framework for this project, a combination of fluorescence microscopy, cytotoxicity assays, live cell imaging, and fluorescence-activated cell sorting was applied to understand the pathogenicity of two Corynebacterium strains isolated from fatal cases of systemic infections. The results showed a clear cytotoxic effect of the bacteria. The observed survival of the pathogens in macrophages and, subsequent, necrotic lysis of cells may be mechanisms explaining dissemination of C. diphtheriae and C. ulcerans to distant organs in the body.
Keyphrases
- endothelial cells
- cell death
- single molecule
- high resolution
- cell cycle arrest
- escherichia coli
- induced pluripotent stem cells
- adipose tissue
- induced apoptosis
- high throughput
- pluripotent stem cells
- cell therapy
- lymph node
- energy transfer
- oxidative stress
- antimicrobial resistance
- mesenchymal stem cells
- gram negative
- biofilm formation