Enzymatic and Cellular Degradation of Carbon-Based Biconcave Nanodisks.
Zhiyong WeiQingxin MuHui WangGuanyou LinMiqin ZhangPublished in: Micromachines (2022)
The assessment of the biodegradability of nanomaterials is of pragmatic importance for understanding the interactions between nanomaterials and biological systems and for the determination of ultimate fate of these materials as well as their potential use. We recently developed carbon-based biconcave nanodisks (CBBNs) serving as a versatile nanocarrier for enhanced accumulation in tumors and combined photothermal-chemotherapy. Here, we investigate both the enzymatic and cellular degradation of CBBNs by monitoring their cellular response with electron microscopy, near-infrared absorbance spectroscopy, and cell viability and oxidative stress assessments. Our results show that CBBNs underwent significant degradation in solutions catalyzed by horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ), or in the presence of macrophage cells. The ability of CBBNs to be degraded in biological systems provides suitability for their future biomedical applications.
Keyphrases
- cell cycle arrest
- hydrogen peroxide
- cell death
- nitric oxide
- electron microscopy
- oxidative stress
- drug delivery
- induced apoptosis
- high resolution
- dna damage
- current status
- study protocol
- randomized controlled trial
- ischemia reperfusion injury
- cancer therapy
- radiation therapy
- climate change
- molecularly imprinted
- endoplasmic reticulum stress
- mass spectrometry
- diabetic rats
- drug release
- heat shock
- human health
- heat stress