Mulberry Polyphenol Extract Inhibits FAK/Src/PI3K Complex and Related Signaling To Regulate the Migration in A7r5 Cells.
Meng-Hsun YuTsung-Yuan YangHsieh-Hsun HoHui-Pei HuangKuei-Chuan ChanChau-Jong WangPublished in: Journal of agricultural and food chemistry (2018)
Atherosclerosis is characterized by the buildup of plaque inside arteries. Our recent studies demonstrated that polyphenolic natural products can reduce oxidative stress, inflammation, angiogenesis, hyperlipidemia, and hyperglycemia. A previous study also showed that mulberry water extract (MWE) can inhibit atherosclerosis and contains considerable amounts of polyphenols. Therefore, in the present study, we investigated whether mulberry polyphenol extract (MPE) containing high levels of polyphenolic compounds could affect vascular smooth muscle cell (VSMC; A7r5 cell) motility. We found that MPE inhibited expression of FAK, Src, PI3K, Akt, c-Raf, and suppressed FAK/Src/PI3K interaction. Further investigations showed that MPE reduced expression of small GTPases (RhoA, Cdc42, and Rac1) to affect F-actin cytoskeleton rearrangement, down-regulated expression of MMP2 and vascular endothelial growth factor (VEGF) mRNA through NFκB signaling, and thereby inhibited A7r5 cell migration. Taken together, these findings highlight MPE inhibited migration in VSMC through FAK/Src/PI3K signaling pathway.
Keyphrases
- cell migration
- oxidative stress
- pi k akt
- signaling pathway
- vascular endothelial growth factor
- induced apoptosis
- cell cycle arrest
- poor prognosis
- tyrosine kinase
- smooth muscle
- endothelial cells
- diabetic rats
- cell proliferation
- cardiovascular disease
- binding protein
- cell therapy
- dna damage
- stem cells
- cell death
- high fat diet
- metabolic syndrome
- staphylococcus aureus
- long non coding rna
- anti inflammatory
- mesenchymal stem cells
- biofilm formation
- mass spectrometry
- escherichia coli
- high speed
- case control