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Oligonucleotide library screening for identification of virus-specific T-cell receptors.

Marthe-Lina WeltersSerena StadlerVasiliki AnastasopoulouLars BullingerMatthias LeisegangThomas KammertönsCarlotta WeltersLeo Hansmann
Published in: European journal of immunology (2024)
We demonstrate an optimized oligonucleotide library-based approach for the identification of virus-reactive T-cell receptors using Epstein-Barr virus as an example. HEK293T served as antigen-presenting cells and were co-cultured with human T cells that were transduced with T-cell receptors in question. T-cell activation was detected by CD137 expression.
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