Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a trans -cleavage with MnO 2 nanosheets.
Renpeng XiaNan OuyangTingting WangYuan ZhuangPeng MiaoPublished in: Chemical communications (Cambridge, England) (2024)
The CRISPR/Cas12a system is accelerated by glutathione-mediated reduction of MnO 2 nanosheets. By monitoring the trans -cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.
Keyphrases
- crispr cas
- label free
- genome editing
- gold nanoparticles
- quantum dots
- high throughput
- reduced graphene oxide
- loop mediated isothermal amplification
- ionic liquid
- dna binding
- real time pcr
- molecularly imprinted
- heavy metals
- metal organic framework
- single molecule
- cell free
- risk assessment
- single cell
- living cells
- mass spectrometry
- water soluble
- fluorescent probe