Absolute quantitation of human wild-type DNAI1 protein in lung tissue using a nanoLC-PRM-MS-based targeted proteomics approach coupled with immunoprecipitation.
Hui WangXiaoyan NiNicholas ClarkKristen RandallLianne BoeglinSudha ChivukulaCaroline WooFrank DeRosaGang SunPublished in: Clinical proteomics (2024)
The results clearly demonstrate that the developed assay can accurately quantitate low-abundance WT DNAI1 protein in human lung tissue with high sensitivity, indicating its high potential use in the drug development for DNAI1 mutation-caused PCD therapy.
Keyphrases
- mass spectrometry
- wild type
- ms ms
- endothelial cells
- protein protein
- multiple sclerosis
- high throughput
- binding protein
- liquid chromatography
- induced pluripotent stem cells
- stem cells
- high performance liquid chromatography
- cancer therapy
- small molecule
- liquid chromatography tandem mass spectrometry
- high resolution
- pluripotent stem cells
- single cell
- risk assessment