Downregulation of SETD5 Suppresses the Tumorigenicity of Hepatocellular Carcinoma Cells.
Mijin ParkByul MoonJong Hwan KimSeung-Jin ParkSeon-Kyu KimKihyun ParkJaehoon KimSeon-Young KimJeong-Hoon KimJung-Ae KimPublished in: Molecules and cells (2022)
Hepatocellular carcinoma (HCC) is an aggressive and incurable cancer. Although understanding of the molecular pathogenesis of HCC has greatly advanced, therapeutic options for the disease remain limited. In this study, we demonstrated that SETD5 expression is positively associated with poor prognosis of HCC and that SETD5 depletion decreased HCC cell proliferation and invasion while inducing cell death. Transcriptome analysis revealed that SETD5 loss downregulated the interferon-mediated inflammatory response in HCC cells. In addition, SETD5 depletion downregulated the expression of a critical glycolysis gene, PKM (pyruvate kinase M1/2), and decreased glycolysis activity in HCC cells. Finally, SETD5 knockdown inhibited tumor growth in xenograft mouse models. These results collectively suggest that SETD5 is involved in the tumorigenic features of HCC cells and that targeting SETD5 may suppress HCC progression.
Keyphrases
- poor prognosis
- induced apoptosis
- cell cycle arrest
- cell death
- long non coding rna
- inflammatory response
- signaling pathway
- stem cells
- gene expression
- cell proliferation
- oxidative stress
- dendritic cells
- dna methylation
- tyrosine kinase
- squamous cell carcinoma
- young adults
- papillary thyroid
- cancer therapy
- genome wide
- mesenchymal stem cells
- drug delivery
- copy number
- toll like receptor
- cell therapy
- binding protein