Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line.

Nityanand Jain Dina Nitisa Valdis Pirsko Inese Cakstina-Dzerve

Published in: BMC molecular and cell biology (2020)

The variance in expression behavior amongst sub-clones highlights the potential need for exercising caution while selecting reference genes for MCF-7. GAPDH-CCSER2-PCBP1 triplet offers a reliable alternative to otherwise traditionally used internal controls for optimizing intra- and inter-assay gene expression differences. Furthermore, we suggest avoiding the use of ACTB, GAPDH and PGK1 as single internal controls.

Keyphrases

gene expression
breast cancer cells
genome wide
poor prognosis
dna methylation
bioinformatics analysis
genome wide identification
binding protein
risk assessment
transcription factor
childhood cancer