H3K27me3 Demethylase UTX Restrains Plasma Cell Formation.
Anna K KaniaMadeline J PriceLou-Ella George-AlexanderDillon G PattersonSakeenah L HicksChristopher D ScharerJeremy M BossPublished in: Journal of immunology (Baltimore, Md. : 1950) (2022)
B cell differentiation is associated with substantial transcriptional, metabolic, and epigenetic remodeling, including redistribution of histone 3 lysine 27 trimethylation (H3K27me3), which is associated with a repressive chromatin state and gene silencing. Although the role of the methyltransferase EZH2 (Enhancer of zeste homolog 2) in B cell fate decisions has been well established, it is not known whether H3K27me3 demethylation is equally important. In this study, we showed that simultaneous genetic deletion of the two H3K27 demethylases UTX and JMJD3 (double-knockout [ Utx fl/fl Jmjd3 fl/fl Cd19 cre/+ ] [dKO]) led to a significant increase in plasma cell (PC) formation after stimulation with the T cell-independent Ags LPS and NP-Ficoll. This phenotype occurred in a UTX-dependent manner as UTX single-knockout mice, but not JMJD3 single-knockout mice, mimicked the dKO. Although UTX- and JMJD3-deficient marginal zone B cells showed increased proliferation, dKO follicular B cells also showed increased PC formation. PCs from dKO mice upregulated genes associated with oxidative phosphorylation and exhibited increased spare respiratory capacity. Mechanistically, deletion of Utx and Jmjd3 resulted in higher levels of H3K27me3 at proapoptotic genes and resulted in reduced apoptosis of dKO PCs in vivo. Furthermore, UTX regulated chromatin accessibility at regions containing ETS and IFN regulatory factor (IRF) transcription factor family motifs, including motifs of known repressors of PC fate. Taken together, these data demonstrate that the H3K27me3 demethylases restrain B cell differentiation.
Keyphrases
- transcription factor
- gene expression
- genome wide
- dna methylation
- dna binding
- genome wide identification
- single cell
- oxidative stress
- dna damage
- dendritic cells
- cell therapy
- cell fate
- signaling pathway
- stem cells
- type diabetes
- metabolic syndrome
- inflammatory response
- endoplasmic reticulum stress
- bone marrow
- copy number
- cell proliferation
- skeletal muscle
- big data
- electronic health record
- adipose tissue