Functional Analysis of a Multiple-Domain CTL15 in the Innate Immunity, Eclosion, and Reproduction of Tribolium castaneum .
Suisui WangHuayi AiYonglei ZhangJingxiu BiHan GaoPeng ChenBin LiPublished in: Cells (2023)
C-type lectin X (CTL-X) plays critical roles in immune defense, cell adhesion, and developmental regulation. Here, a transmembrane CTL-X of Tribolium castaneum , TcCTL15, with multiple domains was characterized. It was highly expressed in the early and late pupae and early adults and was distributed in all examined tissues. In addition, its expression levels were significantly induced after being challenged with pathogen-associated molecular patterns (PAMPs) and bacteria. In vitro, the recombinant TcCTL15 could recognize bacteria through binding PAMPs and exhibit agglutinating activity against a narrow range of bacteria in the presence of Ca 2+ . RNAi-mediated TcCTL15 -knockdown-larvae infected with Escherichia coli and Staphylococcus aureus showed less survival, had activated immune signaling pathways, and induced the expression of antimicrobial peptide genes. Moreover, silencing TcCTL15 caused eclosion defects by impairing ecdysone and crustacean cardioactive peptide receptors (CCAPRs). Suppression of TcCTL15 in female adults led to defects in ovary development and fecundity, accompanied by concomitant reductions in the mRNA levels of vitellogenin ( TcVg) and farnesol dehydrogenase ( TcFDH) . These findings imply that TcCTL15 has extensive functions in developmental regulation and antibacterial immunity. Uncovering the function of TcCTL15 will enrich the understanding of CTL-X in invertebrates. Its multiple biological functions endow the potential to be an attractive target for pest control.
Keyphrases
- escherichia coli
- poor prognosis
- staphylococcus aureus
- cell adhesion
- high glucose
- binding protein
- diabetic rats
- candida albicans
- signaling pathway
- biofilm formation
- drug induced
- genome wide
- long non coding rna
- endothelial cells
- cell proliferation
- cell free
- cystic fibrosis
- klebsiella pneumoniae
- anti inflammatory
- climate change
- silver nanoparticles
- induced apoptosis
- wound healing