Biodegradable Scaffolds for Vascular Regeneration Based on Electrospun Poly(L-Lactide- co -Glycolide)/Poly(Isosorbide Sebacate) Fibers.
Monika Śmiga-MatuszowiczJakub WłodarczykMałgorzata SkorupaDominika Czerwińska-GłówkaKaja FołtaMałgorzata PastusiakMałgorzata Adamiec-OrganiściokMagdalena SkoniecznaRoman Maria TurczynMichał SobotaKatarzyna KrukiewiczPublished in: International journal of molecular sciences (2023)
Vascular regeneration is a complex process, additionally limited by the low regeneration potential of blood vessels. Hence, current research is focused on the design of artificial materials that combine biocompatibility with a certain rate of biodegradability and mechanical robustness. In this paper, we have introduced a scaffold material made of poly(L-lactide- co -glycolide)/poly(isosorbide sebacate) (PLGA/PISEB) fibers fabricated in the course of an electrospinning process, and confirmed its biocompatibility towards human umbilical vein endothelial cells (HUVEC). The resulting material was characterized by a bimodal distribution of fiber diameters, with the median of 1.25 µm and 4.75 µm. Genotyping of HUVEC cells collected after 48 h of incubations on the surface of PLGA/PISEB scaffolds showed a potentially pro-angiogenic expression profile, as well as anti-inflammatory effects of this material. Over the course of a 12-week-long hydrolytic degradation process, PLGA/PISEB fibers were found to swell and disintegrate, resulting in the formation of highly developed structures resembling seaweeds. It is expected that the change in the scaffold structure should have a positive effect on blood vessel regeneration, by allowing cells to penetrate the scaffold and grow within a 3D structure of PLGA/PISEB, as well as stabilizing newly-formed endothelium during hydrolytic expansion.
Keyphrases
- tissue engineering
- drug delivery
- stem cells
- induced apoptosis
- anti inflammatory
- drug release
- endothelial cells
- cell cycle arrest
- wound healing
- endoplasmic reticulum stress
- high resolution
- dna methylation
- cell proliferation
- clinical trial
- cell death
- oxidative stress
- genome wide
- randomized controlled trial
- risk assessment
- study protocol
- vascular endothelial growth factor
- pi k akt
- human health