CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942.
Chun-Hung HuangClaire R ShenHung LiLi-Yu SungMeng-Ying WuYu-Chen HuPublished in: Microbial cell factories (2016)
These data demonstrated that CRISPRi-mediated gene suppression allowed for re-directing the cellular carbon flow, thus paving a new avenue to rationally fine-tune the metabolic pathways in PCC 7942 for the production of biotechnological products.