Role of Chk1 gene in molecular classification and prognosis of gastric cancer using immunohistochemistry and LORD-Q assay.
Nadia SaeedIshrat MahjabeenFarzana HakimMuhammad Zahid HussainAzhar MehmoodAsif NisarMalik Waqar AhmedMahmood Akhtar KayaniPublished in: Future oncology (London, England) (2022)
Purpose: The aim of the current study was to assess the prognostic value of the Chk1 gene in the DNA damage response pathway in gastric cancer (GC). Methods: Expression levels of the Chk1 were measured in 220 GC tumor tissues and adjacent healthy/noncancerous tissues using real-time PCR and immunohistochemical staining. Genomic instability in GC patients was measured using the long-run real-time PCR technique for DNA-damage quantification assay and comet assay. Results: Significantly downregulated expression of Chk1 was observed at the mRNA level (p < 0.0001) and protein level (p < 0.0001). Significantly increased frequency of lesions/10 kb and comets was observed in tumor tissues compared with control tissues. Conclusion: The data suggest that downregulated expression of Chk1 and positive Heliobacter pylori infection status may have prognostic significance in GC.
Keyphrases
- dna damage response
- real time pcr
- poor prognosis
- dna repair
- dna damage
- gene expression
- binding protein
- high throughput
- copy number
- end stage renal disease
- genome wide
- gas chromatography
- ejection fraction
- chronic kidney disease
- oxidative stress
- machine learning
- newly diagnosed
- long non coding rna
- deep learning
- prognostic factors
- peritoneal dialysis
- genome wide identification