A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis .
César Millán-PachecoRodrigo ArreolaAlma Villalobos-OsnayaGeorgina Garza-RamosIris N SerratosAdelaida Díaz-VilchisEnrique Rudiño-PiñeraMaría Elizbeth Alvarez SánchezPublished in: Pathogens (Basel, Switzerland) (2023)
To understand whether protein Tv-PSP1 from Trichomonas vaginalis recognizes mRNA parasite stem-loop structures, we conducted REMSA and intrinsic fluorescence assays. We found the recombinant Tv-PSP1 structure, determined with X-ray crystallography, showed unusual thermal stability of the quaternary structure, associated with a disulfide bridge CYS76-CYS104. To gain deeper insight into the Tv-PSP1 interaction with mRNA stem-loops (mRNAsl) and its relationship with thermal stability, we also used an integrated computational protocol that combined molecular dynamics simulations, docking assays, and binding energy calculations. Docking models allowed us to determine a putative contact surface interaction region between Tv-PSP1 and mRNAsl. We determined the contributions of these complexes to the binding free energy (ΔG b ) in the electrostatic (ΔG elec ) and nonelectrostatic (ΔG non-elec ) components using the Adaptive Poisson-Boltzmann Solver (APBS) program. We are the first, to the best of our knowledge, to show the interaction between Tv-PSP1 and the stem-loop structures of mRNA.
Keyphrases
- molecular dynamics simulations
- binding protein
- high resolution
- molecular docking
- molecular dynamics
- protein protein
- high throughput
- healthcare
- randomized controlled trial
- transcription factor
- quality improvement
- single molecule
- density functional theory
- computed tomography
- dna binding
- small molecule
- mass spectrometry
- single cell
- dual energy
- electron microscopy