Exposure of bisphenol A in breast cancer patients-quantitatively assessed by sensitivity-enhanced high-performance liquid chromatography coupled with fluorescence detection: A case-control study.

A simple, reproducible and sensitive liquid chromatography (HPLC) method has been developed and validated for estimation of bisphenol A (BPA) in human urine. A simple liquid-liquid extraction technique was used in BPA sample preparation. The analyte was chromatographed on a Shimadzu Prominence HPLC system using isocratic mobile phase conditions at a flow rate of 0.500 ml/min and a Hypersil Gold C18 column maintained at 40°C. Quantification was performed on a fluorescence detector set at excitation 275 nm, emission 313 nm and bisphenol B was used as internal standard. The total run time was 8 min. The method was found to have acceptable sensitivity, selectivity, accuracy (98.82-103.64%), precision (1.17-5.36) and stability in the validation experiment carried out as per the USFDA guidelines. The method sensitivity was as low as 0.50 ng/ml. The applicability of the validated analytical method was established in human patient urine samples. The mean human urine BPA concentrations were 1.18 ± 2.11 ng/ml in the control group and 5.76 ± 6.00 ng/ml in the patient group (P < 0.001). Therefore, this method could be considered as an alternative for routine bio-monitoring of BPA which is less expensive and feasible in resource-poor settings.