Oligomeric Procyanidin Nanoliposomes Prevent Melanogenesis and UV Radiation-Induced Skin Epithelial Cell (HFF-1) Damage.
Yashu ChenFenghong HuangDavid Julian McClementsBijun XieZhida SunQianchun DengPublished in: Molecules (Basel, Switzerland) (2020)
The potential protective effect of nanoliposomes loaded with lotus seedpod oligomeric procyanidin (LSOPC) against melanogenesis and skin damaging was investigated. Fluorescence spectroscopy showed that, after encapsulation, the LSOPC-nanoliposomes still possessed strong inhibitory effects against melanogenesis, reducing the activity of both monophenolase and diphenolase. Molecular docking indicated that LSOPC could generate intense interactive configuration with tyrosinase through arene-H, arene-arene, and hydrophobic interaction. An ultraviolet radiated cell-culture model (human foreskin fibroblast cell (HFF-1)) was used to determine the protective effects of the LSOPC-nanoliposomes against skin aging and damage. Results showed that LSOPC-nanoliposomes exerted the highest protective effects against both ultraviolet B (UVB) and ultraviolet A (UVA) irradiation groups compared with non-encapsulated LSOPC and a control (vitamin C). Superoxide dismutase (SOD) and malonaldehyde (MDA) assays demonstrated the protection mechanism may be related to the anti-photooxidation activity of the procyanidin. Furthermore, a hydroxyproline assay suggested that the LSOPC-nanoliposomes had a strong protective effect against collagen degradation and/or synthesis after UVA irradiation.
Keyphrases
- radiation induced
- wound healing
- molecular docking
- soft tissue
- high throughput
- oxidative stress
- water soluble
- radiation therapy
- single molecule
- endothelial cells
- single cell
- drug delivery
- high resolution
- cell therapy
- risk assessment
- stem cells
- hydrogen peroxide
- cancer therapy
- climate change
- breast cancer cells
- cell death
- light emitting
- drug induced
- quantum dots