Therapeutic Potential of Extracts from Macaranga tanarius (MTE) in Diabetic Nephropathy.
Yung-Chien HsuCheng-Chih ChangChing-Chuan HsiehYa-Hsueh ShihHsiu-Ching ChangChun-Liang LinPublished in: Plants (Basel, Switzerland) (2023)
Diabetic nephropathy is a complication of diabetes that leads to end-stage kidney disease and is a major health burden worldwide. Prenylflavonoid compounds extracted from Macaranga tanarius (MTE) exhibit anti-inflammation, anti-oxidant, and anti-bacterial properties. However, the effects of these compounds on diabetic nephropathy remain unclear. The effects of MTE on diabetic nephropathy were investigated in vitro by using mouse renal mesangial cells and in vivo by using a db/db knockout mouse model. No overt alteration in proliferation was observed in mouse renal mesangial cells treated with 0-1 μg/mL MTE. Western blot analysis indicated that MTE dose-dependently attenuated the expression of fibronectin, α-smooth muscle actin, and collagen IV. Administration of MTE ameliorated renal albumin loss in db/db mice. Immunohistochemical staining revealed that MTE mitigated diabetes-induced fibronectin and collagen IV expression. Periodic acid-Schiff (PAS) and trichrome staining also showed that administration of MTE reduced the renal fibrosis phenomenon. MTE significantly ameliorated diabetes-induced nephropathy.
Keyphrases
- diabetic nephropathy
- type diabetes
- induced apoptosis
- smooth muscle
- cardiovascular disease
- mouse model
- poor prognosis
- high glucose
- oxidative stress
- healthcare
- cell cycle arrest
- diabetic rats
- mental health
- endothelial cells
- binding protein
- endoplasmic reticulum stress
- south africa
- skeletal muscle
- social media
- liver fibrosis
- atomic force microscopy
- cell death
- mass spectrometry
- single molecule
- tissue engineering
- health promotion