Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe 3+ in human blood samples and cells.
Vishaka V HManav SaxenaGeetha Balakrishna RSachin LatiyanShilpee JainPublished in: RSC advances (2019)
The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe 3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro . Significantly trace levels of Fe 3+ ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe 3+ . As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe 3+ in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe.
Keyphrases
- fluorescent probe
- living cells
- induced apoptosis
- cell cycle arrest
- quantum dots
- endoplasmic reticulum stress
- signaling pathway
- label free
- aqueous solution
- machine learning
- metal organic framework
- oxidative stress
- risk assessment
- ionic liquid
- loop mediated isothermal amplification
- real time pcr
- induced pluripotent stem cells
- pluripotent stem cells
- case control
- low cost
- energy transfer