CRISPER/CAS System, a Novel Tool of Targeted Therapy of Drug-Resistant Lung Cancer.
Vahid Akbari KordkheyliMohsen RashidiYasaman ShokriSamane FallahpourAtena VarijiEhsan Nabipour GharaSayed Mostafa HosseiniPublished in: Advanced pharmaceutical bulletin (2021)
Lung cancer (LC) is the most common cause of cancer-related death worldwide. Patients with LC are usually diagnosed at advanced phases. Five-year survival rate in LC patients is approximately 16%. Despite decades of research on LC treatments, clinical outcomes are still very poor, necessitating to develop novel technologies to manage the disease. Considering the role of genetic and epigenetic changes in oncogenes and tumor-suppressor genes in cancer progression, gene therapy provides a hot spot in cancer treatment research. Gene therapy offers less side effects compared to conventional methods such as chemotherapy. Unlike the traditional approaches of gene therapy that have temporary effects, using genetic modification tools can offer persistent cure. Over the past a few years, many studies have effectively used the CRISPR-Cas9 approach to modify gene expression in cells. This system is applied to induce site-specific mutagenesis and epigenetic modifications and regulate gene expression. In this review, we discuss recent applications of the CRISPR-Cas9 technology in treating LC.
Keyphrases
- gene therapy
- crispr cas
- gene expression
- genome editing
- drug resistant
- dna methylation
- simultaneous determination
- genome wide
- mass spectrometry
- multidrug resistant
- end stage renal disease
- newly diagnosed
- induced apoptosis
- chronic kidney disease
- solid phase extraction
- acinetobacter baumannii
- copy number
- radiation therapy
- prognostic factors
- tandem mass spectrometry
- squamous cell carcinoma
- signaling pathway
- cell cycle arrest
- transcription factor
- high resolution mass spectrometry
- cell proliferation
- high resolution
- case control
- pi k akt
- free survival