CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines.
Yuichiro KaiboriDaisuke NagakuboPublished in: Cancer gene therapy (2022)
Malignant features such as the acquisition of metastatic ability, stemness of cells, and therapeutic resistance of cancer cells are associated with epithelial-mesenchymal transition (EMT) accompanied by changes in motility and morphology. Recent reports implicated that the formation of polyploid giant cancer cells (PGCCs) in human malignancy correlated with the EMT processes. Chemokines are often involved in the regulation of cancer cell migration into tissues, and various types of human cancers exhibit enhanced expression of chemokine receptors, which could augment intrinsic potentials such as invasive activity, proliferating ability, and survival capacity in cancer cells. Nevertheless, the contribution of CCR3 in malignant cancer cells is controversial because it is a well-known primal receptor for the migration of eosinophils, one of the cells of the innate immune system. Here, we explored the blockage of chemokine receptor CCR3 in carcinoma cell lines and found that inhibition of CCR3 induced the formation of polyploid giant cells and stabilization of β-catenin via the PI3K/Akt/GSK-3β signaling pathway, which are processes associated with EMT. As a result of CCR3 inhibition, converted cells acquired enhanced mobile and proliferation abilities. In summary, these data indicate that modulation of the CCR3/PI3K/Akt/GSK-3β signaling pathway regulates polyploidization associated with the EMT processes.
Keyphrases
- epithelial mesenchymal transition
- signaling pathway
- induced apoptosis
- pi k akt
- cell cycle arrest
- transforming growth factor
- dendritic cells
- regulatory t cells
- cell migration
- cell death
- endoplasmic reticulum stress
- immune response
- oxidative stress
- endothelial cells
- gene expression
- poor prognosis
- squamous cell carcinoma
- long non coding rna
- pseudomonas aeruginosa
- dna methylation
- young adults
- artificial intelligence
- electronic health record
- lymph node metastasis
- staphylococcus aureus
- rare case
- candida albicans