CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
Yuta NihongakiYuichi FuruhataTakahiro OtabeSaki HasegawaKeitaro YoshimotoMoritoshi SatoPublished in: Nature methods (2017)
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.