A Phlorotanin, 6,6'-bieckol from Ecklonia cava, Against Photoaging by Inhibiting MMP-1, -3 and -9 Expression on UVB-induced HaCaT Keratinocytes.
Yuan-Lin HeZhenbang XiaoShengtao YangChunxia ZhouShengli SunPengzhi HongZhong-Ji QianPublished in: Photochemistry and photobiology (2021)
Skin is the outmost layer of human and sustains most of the external UVB irradiation, which possibly causes the skin photoaging. As a natural antioxidant, marine natural products have been paid more and more attention to their positive effects on photoaging. 6,6'-bieckol is a phlorotanin isolated from Ecklonia cava, while its antiphotoaging bioactivity and mechanism have not been clear yet. This study proves that 6,6'-bieckol enhances cells viability and decreases the level of ROS in UVB-induced human immortalized keratinocytes (HaCaT) cells. It also resulted in significant downregulation of matrix metalloproteinases (MMPs), p-c-Fos, phosphorylated JNK, p38, IκB and p65. In addition, molecular docking also showed that 6,6'-bieckol could bind to MMP-1, MMP-3 and MMP-9. Finally, it was proved that 6,6'-bieckol acts on MMPs through the MAPK/AP-1 and NF-κB pathways to reduce UVB-induced oxidative stress damage in HaCaT cells. Therefore, 6,6'-bieckol is a functional food and skin care ingredient with great potential in preventing photoaging.
Keyphrases
- induced apoptosis
- signaling pathway
- oxidative stress
- cell cycle arrest
- molecular docking
- endothelial cells
- pi k akt
- cell death
- wound healing
- high glucose
- endoplasmic reticulum stress
- healthcare
- diabetic rats
- cell migration
- poor prognosis
- cell proliferation
- soft tissue
- inflammatory response
- dna damage
- induced pluripotent stem cells
- anti inflammatory
- nitric oxide
- chronic pain
- lps induced
- transcription factor
- pain management
- hydrogen peroxide
- toll like receptor