Human and murine fibroblast single cell transcriptomics reveals fibroblast clusters are differentially affected by ageing, and serum cholesterol.
Kim van KuijkIan R McCrackenRenée J H A TillieS E J AsselberghsDlzar A KhederS MuitjensHan JinR S TaylorR Wichers SchreurChristoph KuppeRoss DobiePrakash RamachandranMarion J J GijbelsLieve TemmermanPhoebe M KirkwoodJ LuytenYanming LiHeidi NoelsPieter G GoossensJohn R Wilson-KanamoriLeon J SchurgersY H ShenBarend M E MeesErik A L BiessenNeil Cowan HendersonRafael KramannAndrew H BakerJudith C SluimerPublished in: Cardiovascular research (2023)
We provide two adventitial fibroblast-specific markers, PDGFRA and DPEP1, and demonstrate fibroblast heterogeneity in health and cardiovascular disease in humans and mice. Biological relevance is evident from regulation of fibroblast clusters by age and hypercholesterolemia in vivo, associations with human atherosclerotic plaque traits, and enrichment of genes with a GWAS for CVD.
Keyphrases
- single cell
- cardiovascular disease
- endothelial cells
- rna seq
- healthcare
- public health
- genome wide
- wound healing
- induced pluripotent stem cells
- type diabetes
- mental health
- high throughput
- risk assessment
- health information
- adipose tissue
- metabolic syndrome
- cardiovascular risk factors
- transcription factor
- high fat diet induced
- health promotion