Valorization of Wild-Type Cannabis indica by Supercritical CO 2 Extraction and Insights into the Utilization of Raffinate Biomass.

Supercritical CO 2 extraction (SCCO 2 ) extraction of cannabis oil from Indian cannabis ( Cannabis indica ) leaves was optimized through a central composite design using CO 2 pressure (150-250 bar), temperature (30-50 °C) and time (1-2 h). From the regression model, the optimal CO 2 pressure, extraction temperature and time were 250 bar, 43 °C and 1.7 h, respectively resulting in the experimental yield of 4.9 wt% of cannabis oil via SCCO 2 extraction. The extract contained cannabidiol, tetrahydrocannabivarin, Δ 9 -tetrahydrocannabinol and Δ 8 -tetrahydrocannabinol as well as two terpenoids such as cis-caryophyllene and α-humulene. Besides SCCO 2 extraction of cannabis oil, the raffinate biomass was utilized to extract polyphenols using water as the extraction medium. Cannabis oil and water extractive were investigated for their half-maximal inhibitory concentration (IC 50 ) values, which were found to be 1.3 and 0.6 mg/mL, respectively. This is comparable to the commercially available antioxidant such as butylated hydroxytoluene with an IC 50 value of 0.5 mg/mL. This work on SCCO 2 extraction of cannabinoids and other valuable bioactive compounds provides an environmentally sustainable technique to valorize cannabis leaves.